Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional

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Karyotic cell nucleus and endomembrane system as per the chimeric model. The key event in the origin of the eukaryotic cell is postulated to be a symbiotic association between a gram-negative eubacterium (from the proteobacteria-1 group) and likely an "eocyte" archaebacterium. This association led to the loss of the outer membrane from the gram-negative bacterium (not shown). As the membrane of th