Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w

Were aged 80 or above. Data was not available for one patient. Of the 207 patients for whom data was available, the mean age was 84.3 years. Data for 23 of these patients was not available to a year. Of the 133 surgical patients, 49(36.8 ) were admitted after elective and 84(63.2 ) after emergency surgery. At one year, 39 of the elective patients were alive and 31 of the emergency patients. Of t

Were aged 80 or above. Data was not available for one patient. Of the 207 patients for whom data was available, the mean age was 84.3 years. Data for 23 of these patients was not available to a year. Of the 133 surgical patients, 49(36.8 ) were admitted after elective and 84(63.2 ) after emergency surgery. At one year, 39 of the elective patients were alive and 31 of the emergency patients. Of t

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Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10 [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1 (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4 on a rotating wheel at 20 rpm. Beads were

Rianal cellulitisPerianal cellulitis should be promptly recognized in neutropenic patients, as they are associated with significant morbidity and mortality [52]. Necrotizing cellulitis and cellulitis-induced septic shock require surgery. However, surgery is sometimes a complex decision and some authors suggest that, in the absence of septic shock, the management depends on the presence of fluctuat

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