Peptide M (positions 71-90) minimized the signal to .13 ± .02 in comparison to untreated samples and peptide N (76-95) decreased the sign to .21 ± .03 of the untreated emission. In this scenario, illumination at 680 nm qualified prospects to emission involving 520 and 620 nm if donor and acceptor beads are co-localized through conversation of GST-AKAP79 (1-153) and biotin-CaM.