Activate the MAGE-A1 promoter and to which extent, we compared its influence with the stimulatory effect of 5-aza-CdR and/or TSA on MAGE-A1 transcription in cancer cell line settings. For our current investigations, we chose 3 breast cancer cell lines: MDA-MB-468, MCF-7 and BCM1 because of their different levels of MAGE-A1 and BORIS transcripts. As shown in Table 1 and measured by quantitative rea
Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional
Istone modifications at the promoter of MAGE-ABesides DNA methylation, histone modifications also have an impact on promoter activity. In general, acetylation of N-terminal histone tails is a dominant signal for active chromatin facilitating the binding of components of the basal transcription machinery and transcription factors [26]. Histone methylation can be either an active or repressive signa
Istone modifications at the promoter of MAGE-ABesides DNA methylation, histone modifications also have an impact on promoter activity. In general, acetylation of N-terminal histone tails is a dominant signal for active chromatin facilitating the binding of components of the basal transcription machinery and transcription factors [26]. Histone methylation can be either an active or repressive signa
Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional