To convey AKAP79 in microbes in a non-aggregated sort, we cloned the D/D domain (residues 1-45) of human PKA RIIα into pGEX6P1 for expression as a PreScission-cleavable fusion at the N-terminus of GST, and integrated a 2nd cistron that contains N-terminally 6xHis-tagged AKAP79. C-lobe of CaM (residues 85-148, PDB 1IWQ) as the search design.
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