To express AKAP79 in micro organism in a non-aggregated form, we cloned the D/D domain (residues 1-45) of human PKA RIIα into pGEX6P1 for expression as a PreScission-cleavable fusion at the N-terminus of GST, and involved a next cistron that contains N-terminally 6xHis-tagged AKAP79. C-lobe of CaM (residues 85-148, PDB 1IWQ) as the lookup model.