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No other peptides reduced emission beneath .60 relative to untreated samples. Anti-FLAG immunblot of lysates from HEK293T cells utilised in AKAR4 recordings demonstrating relative expression amounts of WT and Δ79-86 FLAG-AKAP79. We also shown that ablating the CaM-binding web-site in AKAP79 improves resting PKA phosphorylation in HEK293T cells. CaM N-lobe is not expected for binding AKAP79.