Peptide M (positions 71-90) decreased the signal to .13 ± .02 in contrast to untreated samples and peptide N (76-95) reduced the sign to .21 ± .03 of the untreated emission. In this circumstance, illumination at 680 nm qualified prospects to emission in between 520 and 620 nm if donor and acceptor beads are co-localized through interaction of GST-AKAP79 (1-153) and biotin-CaM.