Valuated by the Ct method as follows: Ct = Ct value of reference RPLPO - Ct value of mRNA of interest. The relative expression levels of the mRNA of interest corresponded to the 2^(Ct)*1000 value.The statistical analyses were performed using the SPSS software package, version 18.0 (SPSS Inc. Chicago, IL). Statistical difference of mRNA expressions was calculated using ANOVA with Dunnett test for a
Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional
Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional