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No other peptides diminished emission beneath .60 relative to untreated samples. Anti-FLAG immunblot of lysates from HEK293T cells applied in AKAR4 recordings demonstrating relative expression ranges of WT and Δ79-86 FLAG-AKAP79. We also demonstrated that ablating the CaM-binding web site in AKAP79 improves resting PKA phosphorylation in HEK293T cells. CaM N-lobe is not needed for binding AKAP79.

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