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<title>The Meetingpoint at Slavyanski.net / wheeleffect7 / All</title>
<link>https://slavyanski.net/sb2020</link>
<description>Your Source for Social News and Networking</description>
<pubDate>Mon, 31 Aug 2020 11:37:14 +0000</pubDate>
<language>en</language>
<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 31 Aug 2020 11:37:14 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Mon, 31 Aug 2020 11:09:12 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Mon, 31 Aug 2020 10:29:33 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Wed, 26 Aug 2020 11:48:15 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Wed, 26 Aug 2020 11:34:40 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Wed, 26 Aug 2020 11:17:15 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Wed, 26 Aug 2020 10:50:48 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 21 Aug 2020 19:20:11 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 21 Aug 2020 16:03:34 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-2</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-2"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Thu, 20 Aug 2020 18:53:01 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-2</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Thu, 20 Aug 2020 18:04:52 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-7"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Thu, 20 Aug 2020 18:04:17 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-7</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 17 Aug 2020 20:55:16 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-1</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 17 Aug 2020 17:48:13 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-9</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-9"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Fri, 14 Aug 2020 20:20:16 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-9</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-1"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Fri, 14 Aug 2020 16:30:56 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-1</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-8"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 14 Aug 2020 16:00:51 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-8</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Fri, 14 Aug 2020 15:32:17 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-1</guid>
</item>

<item>
	<title><![CDATA[Phosphatase activity of PP2A in studies involving a second known]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known-9</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known-9"><![CDATA[Phosphatase activity of PP2A in studies involving a second known]]></source>
	<description><![CDATA[Phosphatase activity of PP2A in studies involving a second known binding partner of tAg, the JCV agnoprotein [40]. We were surprised to identify two LxCxE motifs that had been overlooked in the unique region of the JCV tAg; one of these sites is also found in BKV but neither site resides in the corresponding SV40, WUV, KIV or MCV polyomavirus proteins. As predicted, JCV tAg binds members of the re ]]></description>
	<pubDate>Wed, 12 Aug 2020 00:54:03 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known-9</guid>
</item>

<item>
	<title><![CDATA[Phosphatase activity of PP2A in studies involving a second known]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known"><![CDATA[Phosphatase activity of PP2A in studies involving a second known]]></source>
	<description><![CDATA[Phosphatase activity of PP2A in studies involving a second known binding partner of tAg, the JCV agnoprotein [40]. We were surprised to identify two LxCxE motifs that had been overlooked in the unique region of the JCV tAg; one of these sites is also found in BKV but neither site resides in the corresponding SV40, WUV, KIV or MCV polyomavirus proteins. As predicted, JCV tAg binds members of the re ]]></description>
	<pubDate>Tue, 11 Aug 2020 23:48:18 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=phosphatase-activity-of-pp2a-in-studies-involving-a-second-known</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Tue, 11 Aug 2020 20:47:35 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 07 Aug 2020 01:35:37 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 07 Aug 2020 01:35:07 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-3</guid>
</item>

<item>
	<title><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a-4"><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A]]></source>
	<description><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A binding is associated with a transformed phenotype. It should be noted that to detect PP2A binding in this study, we employed antibodies that recognized the catalytic subunit of PP2A. PP2A is found abundantly as either a holoenzyme consisting of the AC core plus a regulatory B subunit, or as the AC core alone. High levels  ]]></description>
	<pubDate>Fri, 07 Aug 2020 01:13:29 +0000</pubDate>
	<author>wheeleffect7</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a-4</guid>
</item>

<item>
	<title><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a"><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A]]></source>
	<description><![CDATA[Rvation will require additional investigation to determine if enhanced PP2A binding is associated with a transformed phenotype. It should be noted that to detect PP2A binding in this study, we employed antibodies that recognized the catalytic subunit of PP2A. PP2A is found abundantly as either a holoenzyme consisting of the AC core plus a regulatory B subunit, or as the AC core alone. High levels  ]]></description>
	<pubDate>Fri, 07 Aug 2020 01:13:12 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=rvation-will-require-additional-investigation-to-determine-if-enhanced-pp2a</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-8"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 07 Aug 2020 00:00:17 +0000</pubDate>
	<author>wheeleffect7</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-8</guid>
</item>

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