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<title>The Meetingpoint at Slavyanski.net / coltticket5 / Upvoted</title>
<link>https://slavyanski.net/sb2020</link>
<description>Your Source for Social News and Networking</description>
<pubDate>Fri, 28 Aug 2020 06:32:36 +0000</pubDate>
<language>en</language>
<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 28 Aug 2020 06:32:36 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</guid>
</item>

<item>
	<title><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3"><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of]]></source>
	<description><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of the J domain of the polyomavirus tumor proteins make direct contacts with the Rb proteins and Hsc70, respectively; together they effect the release of members of the E2F family of transcription factors from their Rb partners to promote cell cycle progression [reviewed in 55]. There is less certainty about the identity of tAg amino acids ]]></description>
	<pubDate>Mon, 24 Aug 2020 06:17:53 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3</guid>
</item>

<item>
	<title><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3"><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of]]></source>
	<description><![CDATA[L cycle regulation. The LxCxE motif and the tripeptide HDP of the J domain of the polyomavirus tumor proteins make direct contacts with the Rb proteins and Hsc70, respectively; together they effect the release of members of the E2F family of transcription factors from their Rb partners to promote cell cycle progression [reviewed in 55]. There is less certainty about the identity of tAg amino acids ]]></description>
	<pubDate>Mon, 24 Aug 2020 06:17:16 +0000</pubDate>
	<author>coltticket5</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=l-cycle-regulation-the-lxcxe-motif-and-the-tripeptide-hdp-of-3</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Thu, 20 Aug 2020 11:54:16 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Thu, 20 Aug 2020 10:48:58 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-6</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Thu, 20 Aug 2020 10:48:25 +0000</pubDate>
	<author>coltticket5</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Tue, 18 Aug 2020 13:38:14 +0000</pubDate>
	<author>coltticket5</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-9</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Tue, 18 Aug 2020 11:30:05 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 17 Aug 2020 07:22:14 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Mon, 17 Aug 2020 07:06:53 +0000</pubDate>
	<author>coltticket5</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Mon, 17 Aug 2020 07:05:39 +0000</pubDate>
	<author>coltticket5</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-5</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 14 Aug 2020 12:17:43 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 14 Aug 2020 11:39:12 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-3</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 14 Aug 2020 11:22:37 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-1</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Fri, 14 Aug 2020 11:21:59 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the-4</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 14 Aug 2020 10:35:34 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Thu, 06 Aug 2020 12:57:18 +0000</pubDate>
	<author>coltticket5</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc</guid>
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