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<title>The Meetingpoint at Slavyanski.net / lyre3ball / Upvoted</title>
<link>https://slavyanski.net/sb2020</link>
<description>Your Source for Social News and Networking</description>
<pubDate>Fri, 28 Aug 2020 05:36:11 +0000</pubDate>
<language>en</language>
<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 28 Aug 2020 05:36:11 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Fri, 28 Aug 2020 04:45:36 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Fri, 28 Aug 2020 04:20:07 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Fri, 28 Aug 2020 04:19:50 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-3</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Mon, 24 Aug 2020 04:26:07 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Fri, 21 Aug 2020 05:04:13 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-5</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Thu, 20 Aug 2020 08:04:57 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-6</guid>
</item>

<item>
	<title><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4"><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc]]></source>
	<description><![CDATA[S where phenotyped on MS medium containing 0  and 3  (w/v) Suc and soil (peat/perlite/vermiculite) according to the parameters described previously (Boyes et al., 2001). Seeds were sterilized with chlorine gas and sown on MS medium containing 3  (w/v) Suc followed by 48 h of stratification at 4 . Plants were grown for 2 weeks at 22  with a light intensity of 80 mmol quanta m22 s21 in a 16-h photop ]]></description>
	<pubDate>Thu, 20 Aug 2020 07:35:53 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=s-where-phenotyped-on-ms-medium-containing-0-and-3-wv-suc-4</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Wed, 19 Aug 2020 06:43:16 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-7</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Tue, 18 Aug 2020 09:57:16 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Tue, 18 Aug 2020 09:25:25 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-8</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 17 Aug 2020 05:55:04 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-2</guid>
</item>

<item>
	<title><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4"><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha]]></source>
	<description><![CDATA[Flanked by Gateway recombination cassettes (Supplemental Table S4) and cloned intoMurcha et al.pDONR201. LR reactions were carried out with C-terminal GFP fusion vectors for GFP localization (Carrie et al., 2009), pDEST14 for in vitro transcription and translation, and pB2GW7 (35S CaMV promoter) for Agrobacterium tumefaciensmediated complementation (Karimi et al., 2002). Yeast two-hybrid vectors w ]]></description>
	<pubDate>Mon, 17 Aug 2020 05:28:14 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=flanked-by-gateway-recombination-cassettes-supplemental-table-s4-and-cloned-intomurcha-4</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Mon, 17 Aug 2020 04:51:36 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Fri, 14 Aug 2020 08:49:12 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-5</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-6"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Wed, 12 Aug 2020 17:40:04 +0000</pubDate>
	<author>lyre3ball</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-6</guid>
</item>

<item>
	<title><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the"><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the]]></source>
	<description><![CDATA[On kit (Promega); 10 mL of radiolabeled protein was added to the reaction, and samples were resolved by SDS-PAGE, dried, and exposed to a phosphor-imaging screen.Plant Material and Growth ConditionsArabidopsis (Arabidopsis thaliana) T-DNA insertional knockout lines for Tric1 (SALK_031707, SALK_112126, and At3g49560) and Tric2 (SALK_136525, SALK_149871, and At5g24650) were obtained from ABRC and sc ]]></description>
	<pubDate>Thu, 06 Aug 2020 11:11:20 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=on-kit-promega-10-ml-of-radiolabeled-protein-was-added-to-the</guid>
</item>

<item>
	<title><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-2</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-2"><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM]]></source>
	<description><![CDATA[Mitochondrial pellet was resuspended in digitonin buffer (30 mM HEPES-KOH, pH 7.4, 150 mM potassium acetate, 10  [v/v] glycerol, and 1 mg of digitonin) and kept on ice for 30 min. Ten microliters of antibody serum, 50 mL of Protein A-Sepharose, 1  (w/v) BSA, and Complete protease inhibitor (Roche) were added to the mitochondria and incubated for 6 h at 4  on a rotating wheel at 20 rpm. Beads were  ]]></description>
	<pubDate>Thu, 06 Aug 2020 10:11:47 +0000</pubDate>
	<author>lyre3ball</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=mitochondrial-pellet-was-resuspended-in-digitonin-buffer-30-mm-hepes-koh-ph-7-4-150-mm-2</guid>
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