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<title>The Meetingpoint at Slavyanski.net / pair8health / Upvoted</title>
<link>https://slavyanski.net/sb2020</link>
<description>Your Source for Social News and Networking</description>
<pubDate>Fri, 28 Aug 2020 08:22:26 +0000</pubDate>
<language>en</language>
<item>
	<title><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4"><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></source>
	<description><![CDATA[L line BCM1, the expression of both genes is opposite: no levels of MAGE-A1 [2^(Ct) 1.07] and high levelsof BORIS [2^(Ct) 24.39]. We transiently transfected expression plasmid encoding BORIS into both cell lines, with negligible transcript levels of MAGE-A1, and quantified endogenous MAGE-A1 mRNA by RT (reverse transcription)-PCR and gel electrophoresis. As depicted in Figure 1, BORIS was able to  ]]></description>
	<pubDate>Fri, 28 Aug 2020 08:22:26 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4</guid>
</item>

<item>
	<title><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-5"><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></source>
	<description><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional ]]></description>
	<pubDate>Fri, 21 Aug 2020 23:13:22 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-5</guid>
</item>

<item>
	<title><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-7"><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></source>
	<description><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional ]]></description>
	<pubDate>Thu, 20 Aug 2020 00:01:22 +0000</pubDate>
	<author>pair8health</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-7</guid>
</item>

<item>
	<title><![CDATA[Nhibitor TSA or after transient transfection of these cells with an]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-1"><![CDATA[Nhibitor TSA or after transient transfection of these cells with an]]></source>
	<description><![CDATA[Nhibitor TSA or after transient transfection of these cells with an expression plasmid encoding for BORIS were separated on an agarose gel. The bar chart shows the relative changes in mRNA expression levels of MAGE-A1 in MCF-7 cells by quantitative real-time PCR. The significant p-values are shown (C). H2O lane serves as a negative control. The housekeeping gene -Actin was selected as an internal  ]]></description>
	<pubDate>Wed, 19 Aug 2020 22:52:24 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-1</guid>
</item>

<item>
	<title><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-6"><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></source>
	<description><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional ]]></description>
	<pubDate>Mon, 17 Aug 2020 12:56:57 +0000</pubDate>
	<author>pair8health</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-6</guid>
</item>

<item>
	<title><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4"><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></source>
	<description><![CDATA[L line BCM1, the expression of both genes is opposite: no levels of MAGE-A1 [2^(Ct) 1.07] and high levelsof BORIS [2^(Ct) 24.39]. We transiently transfected expression plasmid encoding BORIS into both cell lines, with negligible transcript levels of MAGE-A1, and quantified endogenous MAGE-A1 mRNA by RT (reverse transcription)-PCR and gel electrophoresis. As depicted in Figure 1, BORIS was able to  ]]></description>
	<pubDate>Thu, 13 Aug 2020 13:01:51 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-4</guid>
</item>

<item>
	<title><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-5"><![CDATA[L line BCM1, the expression of both genes is opposite: no]]></source>
	<description><![CDATA[L line BCM1, the expression of both genes is opposite: no levels of MAGE-A1 [2^(Ct) 1.07] and high levelsof BORIS [2^(Ct) 24.39]. We transiently transfected expression plasmid encoding BORIS into both cell lines, with negligible transcript levels of MAGE-A1, and quantified endogenous MAGE-A1 mRNA by RT (reverse transcription)-PCR and gel electrophoresis. As depicted in Figure 1, BORIS was able to  ]]></description>
	<pubDate>Thu, 13 Aug 2020 13:00:48 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=l-line-bcm1-the-expression-of-both-genes-is-opposite-no-5</guid>
</item>

<item>
	<title><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-4"><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates]]></source>
	<description><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates MAGE-A1 mRNA expression in MCF-7 and BCM1 cellsWe previously demonstrated that the demethylating agent 5-aza-CdR and the histone deacetylase inhibitor TSA synergistically upregulate MAGE-A1 expression in cell lines derived from different cancer types [6]. Moreover, Vatolin et al. reported that conditional ]]></description>
	<pubDate>Mon, 10 Aug 2020 21:48:49 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-4</guid>
</item>

<item>
	<title><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-3"><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates]]></source>
	<description><![CDATA[Aterial, or human data, or used regulated vertebrates or invertebratesResultsBORIS stimulates MAGE-A1 mRNA expression in MCF-7 and BCM1 cellsWe previously demonstrated that the demethylating agent 5-aza-CdR and the histone deacetylase inhibitor TSA synergistically upregulate MAGE-A1 expression in cell lines derived from different cancer types [6]. Moreover, Vatolin et al. reported that conditional ]]></description>
	<pubDate>Mon, 10 Aug 2020 21:48:20 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=aterial-or-human-data-or-used-regulated-vertebrates-or-invertebratesresultsboris-stimulates-3</guid>
</item>

<item>
	<title><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-8</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-8"><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by]]></source>
	<description><![CDATA[Ion levels of BORIS in MDA-MB-468, MCF-7 and BCM1 cells by RT-PCR and gel electrophoresis. As expected, we found a similar expression profile of BORIS mRNA (Figure 2) to that detected by quantitative real-time PCR (Table 1). However, gel electrophoresis and quantitative real time showed no and low expression levels of BORIS in MCF-7 cells, respectively, but the tendency was similar. The additional ]]></description>
	<pubDate>Fri, 07 Aug 2020 21:33:24 +0000</pubDate>
	<author>pair8health</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ion-levels-of-boris-in-mda-mb-468-mcf-7-and-bcm1-cells-by-8</guid>
</item>

<item>
	<title><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the"><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></source>
	<description><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the BORIS mRNA expression nearly completely in presence and absence of scramble shRNA (Figure 3D, p = 0.0001). Likewise, the downregulation of MAGE-A1 expression by BORIS-specific shRNA was more prominent in MCF-7 cells than in MDA-MB-468 cells. As measured by quantitative real time PCR, BORIS-specific shRNA reduced the  ]]></description>
	<pubDate>Fri, 07 Aug 2020 20:36:27 +0000</pubDate>
	<author>pair8health</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the</guid>
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