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<title>The Meetingpoint at Slavyanski.net / dryer1yard / All</title>
<link>https://slavyanski.net/sb2020</link>
<description>Your Source for Social News and Networking</description>
<pubDate>Thu, 27 Aug 2020 13:15:21 +0000</pubDate>
<language>en</language>
<item>
	<title><![CDATA[Activate the MAGE-A1 promoter and to which extent, we compared its]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=activate-the-mage-a1-promoter-and-to-which-extent-we-compared-its-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=activate-the-mage-a1-promoter-and-to-which-extent-we-compared-its-6"><![CDATA[Activate the MAGE-A1 promoter and to which extent, we compared its]]></source>
	<description><![CDATA[Activate the MAGE-A1 promoter and to which extent, we compared its influence with the stimulatory effect of 5-aza-CdR and/or TSA on MAGE-A1 transcription in cancer cell line settings. For our current investigations, we chose 3 breast cancer cell lines: MDA-MB-468, MCF-7 and BCM1 because of their different levels of MAGE-A1 and BORIS transcripts. As shown in Table 1 and measured by quantitative rea ]]></description>
	<pubDate>Thu, 27 Aug 2020 13:15:21 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=activate-the-mage-a1-promoter-and-to-which-extent-we-compared-its-6</guid>
</item>

<item>
	<title><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-1</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-1"><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></source>
	<description><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the BORIS mRNA expression nearly completely in presence and absence of scramble shRNA (Figure 3D, p = 0.0001). Likewise, the downregulation of MAGE-A1 expression by BORIS-specific shRNA was more prominent in MCF-7 cells than in MDA-MB-468 cells. As measured by quantitative real time PCR, BORIS-specific shRNA reduced the  ]]></description>
	<pubDate>Fri, 21 Aug 2020 07:30:55 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-1</guid>
</item>

<item>
	<title><![CDATA[Ults show that changes in the BORIS transcript levels are associated]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ults-show-that-changes-in-the-boris-transcript-levels-are-associated-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ults-show-that-changes-in-the-boris-transcript-levels-are-associated-5"><![CDATA[Ults show that changes in the BORIS transcript levels are associated]]></source>
	<description><![CDATA[Ults show that changes in the BORIS transcript levels are associated with those of MAGE-A1 and corroborate that BORIS is involved in the activation of MAGE-A1 gene expression.BORIS affects the DNA methylation pattern of MAGE-A1 geneFigure 2 BORIS mRNA expression in MDA-MB-468, MCF-7 and BCM1 cells, untreated or treated with 5-aza-CdR. RT-PCR products of BORIS mRNA were separated on an agarose gel. ]]></description>
	<pubDate>Wed, 19 Aug 2020 08:56:59 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ults-show-that-changes-in-the-boris-transcript-levels-are-associated-5</guid>
</item>

<item>
	<title><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-4</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-4"><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not]]></source>
	<description><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not express MAGE-A1 mRNA (Table 1). Based on the usually low transfection efficiency we sorted the transfected MCF-7 cells from untransfected cells by FACS and observed a transfection efficiency of about 10 . Subsequently, sodium bisulfite mapping showed a demethylation of the MAGE-A1 promoter of approximately 56  (range from 44 to 6 ]]></description>
	<pubDate>Wed, 19 Aug 2020 08:28:39 +0000</pubDate>
	<author>dryer1yard</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-4</guid>
</item>

<item>
	<title><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-7</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-7"><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not]]></source>
	<description><![CDATA[E experiments, we chose, therefore, MCF-7 cells, because they do not express MAGE-A1 mRNA (Table 1). Based on the usually low transfection efficiency we sorted the transfected MCF-7 cells from untransfected cells by FACS and observed a transfection efficiency of about 10 . Subsequently, sodium bisulfite mapping showed a demethylation of the MAGE-A1 promoter of approximately 56  (range from 44 to 6 ]]></description>
	<pubDate>Wed, 19 Aug 2020 08:27:27 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=e-experiments-we-chose-therefore-mcf-7-cells-because-they-do-not-7</guid>
</item>

<item>
	<title><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-5</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-5"><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the]]></source>
	<description><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the BORISSchwarzenbach et al. BMC Cancer 2014, 14:796 http://www.biomedcentral.com/1471-2407/14/Page 7 ofAMAGE-A1 429 bp _ ?Actin 202 bp _MCF-BMAGE-A1 429 bp _ ?Actin 202 bp _BCMMAGE-A1 mRNA Expression ( )C80000 60000 40000 20000 2000 1500 1000 500MCF-p=0.p=0.p=0.001 p=0.basalAZATSAAZA + TSABorisFigure 1 Comparison of the MAG ]]></description>
	<pubDate>Wed, 19 Aug 2020 07:57:19 +0000</pubDate>
	<author>dryer1yard</author>
	<category></category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-5</guid>
</item>

<item>
	<title><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-3</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-3"><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the]]></source>
	<description><![CDATA[Ontrol. At 48 or 72 hour post-transfection, we quantified the changes in the BORISSchwarzenbach et al. BMC Cancer 2014, 14:796 http://www.biomedcentral.com/1471-2407/14/Page 7 ofAMAGE-A1 429 bp _ ?Actin 202 bp _MCF-BMAGE-A1 429 bp _ ?Actin 202 bp _BCMMAGE-A1 mRNA Expression ( )C80000 60000 40000 20000 2000 1500 1000 500MCF-p=0.p=0.p=0.001 p=0.basalAZATSAAZA + TSABorisFigure 1 Comparison of the MAG ]]></description>
	<pubDate>Wed, 19 Aug 2020 07:56:28 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=ontrol-at-48-or-72-hour-post-transfection-we-quantified-the-changes-in-the-3</guid>
</item>

<item>
	<title><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-6"><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the]]></source>
	<description><![CDATA[Upregulate MAGE-A1 expression in this cell line. BORIS-specific shRNA reduced the BORIS mRNA expression nearly completely in presence and absence of scramble shRNA (Figure 3D, p = 0.0001). Likewise, the downregulation of MAGE-A1 expression by BORIS-specific shRNA was more prominent in MCF-7 cells than in MDA-MB-468 cells. As measured by quantitative real time PCR, BORIS-specific shRNA reduced the  ]]></description>
	<pubDate>Sat, 15 Aug 2020 09:50:01 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=upregulate-mage-a1-expression-in-this-cell-line-boris-specific-shrna-reduced-the-6</guid>
</item>

<item>
	<title><![CDATA[Nhibitor TSA or after transient transfection of these cells with an]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-6</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-6"><![CDATA[Nhibitor TSA or after transient transfection of these cells with an]]></source>
	<description><![CDATA[Nhibitor TSA or after transient transfection of these cells with an expression plasmid encoding for BORIS were separated on an agarose gel. The bar chart shows the relative changes in mRNA expression levels of MAGE-A1 in MCF-7 cells by quantitative real-time PCR. The significant p-values are shown (C). H2O lane serves as a negative control. The housekeeping gene -Actin was selected as an internal  ]]></description>
	<pubDate>Mon, 10 Aug 2020 02:33:19 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-or-after-transient-transfection-of-these-cells-with-an-6</guid>
</item>

<item>
	<title><![CDATA[Nhibitor TSA on the mRNA expression of MAGE-A1 gene and the]]></title>
	<link>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-on-the-mrna-expression-of-mage-a1-gene-and-the</link>
	<source url="https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-on-the-mrna-expression-of-mage-a1-gene-and-the"><![CDATA[Nhibitor TSA on the mRNA expression of MAGE-A1 gene and the]]></source>
	<description><![CDATA[Nhibitor TSA on the mRNA expression of MAGE-A1 gene and the other family members (MAGE-A2, -A3 and -A12) in different cell lines. Moreover, we assessed the methylation status of the MAGE-A promoters by sodium bisulfite mappingbefore and after stimulation with the demethylating agent 5-aza-CdR and/or TSA. While the methylation patterns clearly correlated with the basal MAGE RNA transcript levels, u ]]></description>
	<pubDate>Fri, 07 Aug 2020 04:59:10 +0000</pubDate>
	<author>dryer1yard</author>
	<category>News</category>
	<votes>1</votes>
	<guid>https://slavyanski.net/sb2020/story.php?title=nhibitor-tsa-on-the-mrna-expression-of-mage-a1-gene-and-the</guid>
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