No other peptides decreased emission beneath .60 relative to untreated samples. Anti-FLAG immunblot of lysates from HEK293T cells used in AKAR4 recordings exhibiting relative expression degrees of WT and Δ79-86 FLAG-AKAP79. We also demonstrated that ablating the CaM-binding web site in AKAP79 boosts resting PKA phosphorylation in HEK293T cells. CaM N-lobe is not needed for binding AKAP79.