No other peptides lowered emission underneath .60 relative to untreated samples. Anti-FLAG immunblot of lysates from HEK293T cells used in AKAR4 recordings displaying relative expression degrees of WT and Δ79-86 FLAG-AKAP79. We also shown that ablating the CaM-binding internet site in AKAP79 will increase resting PKA phosphorylation in HEK293T cells. CaM N-lobe is not required for binding AKAP79.